Applied Biosystems™ BigDye™ M13 HiRes Primers

Catalog No.	Quantity
43-369-38	100 Reactions
43-369-39	1000 Reactions

Catalog No. 43-369-38 Supplier Applied Biosystems™ Supplier No. 4336938

Description

The HI-Res Sequencing Primers are optimized primers designed for use with the BigDye™ Terminator BDT Plus Cycle Sequencing Kit to improve sequencing resolution and data quality, particularly at the 5' (five-prime) end of reads.

The HI-Res Sequencing Primers are optimized primers designed for use with the BigDye™ Terminator BDT Plus Cycle Sequencing Kit to improve sequencing resolution and data quality, particularly at the 5' (five-prime) end of reads. Unlike traditional approaches that require dedicated workflows and alternative polymers, HI-Res primers enable enhanced 5' end resolution within standard sequencing workflows using POP-7™ polymer.

Key Features and Benefits

• Enhance 5' end resolution within standard sequencing workflows using POP-7™ polymer
• Eliminate the need for dedicated workflows or alternative polymers such as POP-6™
• Maintain existing workflows with compatibility across BigDye™ Terminator protocols and CE instruments

Product Details

HI-Res Sequencing Primers are specifically engineered to improve sequencing performance at the 5' end of reads, a region that has traditionally been difficult to resolve with standard primer designs. By optimizing primer characteristics and sequencing signal behavior, these primers enable clearer peak resolution and more reliable base calling in early read regions.

Historically, achieving improved 5' end resolution required modified workflows using alternative polymers such as POP-6™, often introducing additional complexity, time, and verification requirements. HI-Res primers enhance 5' end resolution within standard sequencing workflows using POP-7™ polymer.

These primers are designed to work in conjunction with the BigDye™ Terminator BDT Plus Cycle Sequencing Kit, where the combination of optimized primer design and advanced enzyme chemistry enables improved sequencing performance across a wide range of sample types. This integrated system supports consistent results, even when working with challenging templates such as GC-rich regions or structurally complex sequences.

HI-Res primers can be incorporated into current Sanger sequencing workflows. Their compatibility with standard capillary electrophoresis systems and cleanup methods enables easy adoption while enabling improvements in 5' end resolution.

By enabling improved 5' end resolution within existing workflows, HI-Res primers help laboratories generate higher-quality data, simplify operations, and increase overall sequencing efficiency.

Specifications

• Format: Tube
• Isolation Technology: Magnetic Bead
• Sequencing Type: Genome and DNA Sequencing
• For Use With (Equipment): SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
• For Use With (Application): Sanger Sequencing, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing
• Product Line: BigDye Terminator
• Product Type: Sequencing Kit
• Quantity: 100 Reactions
• Technique: Fluorescent Dye Terminator Sequencing
• Template Compatibility: BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons

For Research Use Only.