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  6. Invitrogen™ Biotin Monoclonal Antibody (BK-1/39), Alexa Fluor™ 488, eBioscience™ 

Invitrogen™ Biotin Monoclonal Antibody (BK-1/39), Alexa Fluor™ 488, eBioscience™
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Catalog No. p-7167697
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100 μg
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Catalog No. Quantity
50-112-9103 100 μg
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Catalog No. 50-112-9103 Supplier Invitrogen™ Supplier No. 53989582
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Description

Mouse Monoclonal Antibody

Description: This BK-1/39 monoclonal antibody reacts with biotin, a B-complex vitamin. As a coenzyme, biotin is involved in the metabolism of fatty acids, amino acids, and gluconeogenesis. In the laboratory, biotin is used to tag molecules of interest for biochemical or cell biological studies. This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. Applications Reported: This BK-1/39 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BK-1/39 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained with a biotin-conjugated antibody. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Biotin
Applications Flow Cytometry
Classification Monoclonal
Clone BK-1/39
Concentration 0.5 mg/mL
Conjugate Alexa Fluor 488
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias Vitamin B4
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
Target Molecule Chemical
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