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Enzo Life Sciences Ubiquitin, (biotinylated) (100 µg)

Supplier: Enzo Life Sciences BMLUW87050100

Biotinylated ubiquitin (prepared by the action of biotinamidocaproic acid N-hydroxysuccinimide ester on ubiquitin) is useful for studying ubiquitinylation in vitro, where it represents an alternative to radio-iodinated ubiquitin. This product contains a number of multiply biotinylated ubiquitin species, which has proven itself to be a reagent of considerable utility particularly for carrying out E1/E2-mediated thiolester assays. Formulation: Lyophilized from Acetonitrile:water (1:1). Appearance: White solid. Source: Produced in E. coli. Solubility: In order to ensure full solubility it is recommended that the material be dissolved in 5µL DMSO prior to dilution with the buffer of choice to the desired working. Concentration. UniProt: P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52). Handling: Avoid freeze/thaw cycles. Long Term Storage: -20°C
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