R&D Systems™ Biotinylated Recombinant Human VEGF 165 Protein

Description

Biotinylated via amines

When Recombinant Human VEGFR1/Flt-1 Fc Chimera Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 321-FL ) is immobilized at 0.2 μg/mL (100 μL/well), Biotinylated Recombinant Human VEGF 165 (Catalog # BT10543) binds with an ED50 of 0.8-6.4 ng/mL.

Specifications

• Accession Number: NP_001165097.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): M.W.-observed: 22-27 kDa, under reducing conditions, M.W.-theoretical: 19 kDa
• Quantity: 25 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein Ala27-Arg191
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
• Conjugate: Biotin
• Reconstitution: Reconstitute at 250 μg/mL in 4 mM HCl.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: VEGF