Invitrogen™ Blasticidin S Deaminase Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143657

Catalog No. PIPA5143657

Description

The antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.

Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week.

Specifications

• Antigen: Blasticidin S Deaminase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BAD
• Gene Alias: blasticidin; BSD
• Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase* from Aspergillus Terreus.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Fungi
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Concentration: ∼0.25 mg/mL
• Formulation: PBS with 1 mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q61337
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12015
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid