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STEMCELL Technologies (-)-Blebbistatin, Size: 25 mg
Supplier: STEMCELL Technologies 72404
(-)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases (Kovács et al.; Straight et al.). It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC₅₀ = 0.5-5.0 μM), while poorly inhibiting smooth muscle myosin (IC₅₀ = 80 μM) (Limouze et al.).MAINTENANCE AND SELF-RENEWAL· Increases human pluripotent stem cell (hPSC) survival and cloning efficiency after dissociation to single cells, downstream of ROCK inhibition (Chen et al.; Ohgushi et al.; Walker et al.; Xu et al.). · Enables hPSC to be cultured on microcarriers without surface coating (Chen et al.)· Inhibits differentiation of human mesenchymal stem cells (McBeath et al.; Engler et al.).
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