Blimp-1 Rat anti-Mouse, PE, Clone: 5E7, eBioscience™ 

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2467	Invitrogen 12985080	25 μg
50-112-2468	Invitrogen 12985082	100 μg

Description

Description: This 5E7 monoclonal antibody recognizes mouse B lymphocyte-induced maturation protein-1 (Blimp-1), which is also known as Prdm1. Blimp-1 is a transcriptional repressor with five zinc finger domains. Blimp-1 is expressed by terminally differentiating B and T cells. Blimp-1 is also expressed by some dendritic cells, and some NK cells. This transcriptional repressor is required for B cell differentiation into plasma cells and serves as a critical antagonist of CD4+ follicular helper T cell differentiation. While Blimp-1 is not required for priming and development of T and B cell effector function, it is critical for effector cell terminal differentiation. Blimp-1 promotes effector molecule secretion while suppressing proliferation. Blimp-1 also acts as a transcriptional antagonist of Bcl-6 and inhibits genes that are related to memory cell differentiation. Applications Reported: The 5E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: The 5E7 antibody has been tested by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Specifications

• Antigen: Blimp-1
• Target Species: Mouse
• Isotype: IgG2a, kappa
• Monoclonal or Polyclonal: Monoclonal
• Format: Liquid
• Type: Antibody
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!

• Clone: 5E7
• Conjugate: PE
• Applications: Flow Cytometry
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

For Research Use Only.