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Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Manufacturer:  Thermo Scientific™ 37530

Catalog No. PI37530



Thermo Scientific™ Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for using in Western blotting, ELISA, immunohistochemistry and other detection methods.


  • Popular – nonfat milk has been used for many years in a variety of protein methods, although it is not recommended for avidin-based techniques because it contains some endogenous biotin

  • Convenient – supplied as a ready-to-use 1X TBS solution; can be diluted as needed

  • Easy to use – formulated with anti-foaming agent and thimerosal-free preservative

  • Flexible – may be used for multiple applications, including as a diluent for antibody

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ELISA, Western Blot
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