Thermo Scientific™ Blocker™ BLOTTO in TBS

Catalog No.	Quantity
PI37530	1 L

Catalog No. PI37530 Supplier Thermo Scientific™ Supplier No. 37530

Description

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific™ Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for using in Western blotting, ELISA, immunohistochemistry and other detection methods.

Highlights:

• Popular – nonfat milk has been used for many years in a variety of protein methods, although it is not recommended for avidin-based techniques because it contains some endogenous biotin

• Convenient – supplied as a ready-to-use 1X TBS solution; can be diluted as needed

• Easy to use – formulated with anti-foaming agent and thimerosal-free preservative

• Flexible – may be used for multiple applications, including as a diluent for antibody

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Specifications

• Chemical Name or Material: Blocker BLOTTO
• Concentration: 1X
• For Use With (Application): Western Blot
• Physical Form: Liquid
• Product Line: Blocker
• Quantity: 1 L

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.