Thermo Scientific™ Blocker™ Casein

Catalog No.	Quantity	Chemical Name or Material
PI37528	1 L	PBS
PI37582	100 mL	PBS
PI37583	100 mL	TBS
FER37528X3	3 x 1 L	PBS
PI37532	1 L	TBS

Catalog No. PI37528 Supplier Thermo Scientific™ Supplier No. 37528

Description

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods.

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

Features of Blocker Casein include:

• Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
• Easy to use—1% casein solution is ready to use; can be diluted further as needed
• Safe—stable, thimerosal-free formulation

Specifications

• Chemical Name or Material: PBS
• Concentration: 1X
• For Use With (Application): Western Blot
• Physical Form: Liquid
• Product Line: Blocker
• Quantity: 1 L

Frequently Asked Questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

What is the main ingredient in Blocker Casein (Cat. No. 37582, 37528, 37528X3)?

Blocker Casein (Cat. No. 37582, 37528, 37528X3) is a ready-to-use, 1% (w/v) solution of purified casein in phosphate-buffered saline (PBS). It is a stable, thimerosal-free formulation and is already at the optimal concentration for most applications. However, it can be diluted further if needed.