MilliporeSigma™ Blood Vessel Staining Kit

Description

Includes

• Rabbit anti-vWF Polyclonal Antibody: One vial containing 100μg at 1mg/mL
• Mouse anti-CD31 Monoclonal Antibody: One vial containing 100μL at 15mg/mL (total protein including IgG and Carrier protein)
• Goat anti-Rabbit Secondary Antibody: One 15mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein
• Goat anti-Mouse Secondary Antibody: One 15mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein
• Blocking Reagent: One 15mL ready-to-use solution containing normal goat serum in PBS with a carrier protein
• Streptavidin-HRP: One 15ml ready-to-use solution of Streptavidin-HRP diluted in Tris-buffered saline (TBS)
• DAB Chromogen A: One 5mL bottle of 3, 3' Diaminobenzidine diluted in TBS
• DAB Chromogen B: Two 12mL bottles of hydrogen peroxide diluted in distilled water
• 20X Rinse Buffer: One 275mL bottle of 20X TBS

Very sensitive immunohistochemical tool for the detection of vascular endothelial cells and provides simple, all-in-one method for staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues

• Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date
• ECM590 detects vWF and CD31 in human paraffin embedded tissues
• In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31

Specifications

• Content And Storage: Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight
• Detection Method: Chromogenic
• For Use With (Application): Immunohistochemistry, Immunohistochemistry (Paraffin)
• Quantity: 1 Kit