Novus Biologicals™ Blu12 Prestained Protein Ladder

Description

Prestained Protein Ladder

The Blu12 Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Specifications

• Formulation: 20 mM Tris-phosphate (pH 7.5), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
• For Use With (Application): 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μL per well for general Western transferring.
• Quantity: 500 μL