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Invitrogen™ Human BMP-5 ELISA Kit

Catalog No. EHBMP5
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests

ELISA

The Human Bone Morphogenic Protein 5 (Hu BMP-5) ELISA quantitates Hu BMP-5 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BMP-5. Principle of the method The Human BMP-5 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TGF-ß family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth, and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling, and maintenance of a variety of other tissues and organs. BMP-5 is expressed in the nervous system, lungs and liver. It is a known regulator for dendritic growth in sympathetic neurons. BMP-5 is a 454 amino acid precursor protein that is cleaved to release the biologically active C-terminal mature protein.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P22003
Assay Range 0.246 to 60 ng/mL
Assay Sensitivity 250 pg/mL
Calibration Range 0.25 ng/mL to 60 ng/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Growth Factors & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 653
Gene Symbol BMP5
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Angiogenesis
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym N/A
Target BMP5
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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