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BPDE-DNA Monoclonal Antibody (5D11), Invitrogen™

Catalog No. 01676388
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100 μg
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Catalog No. Quantity
01-676-388 100 μg
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Catalog No. 01-676-388 Supplier Thermo Scientific Supplier No. MA140269

Description

Mouse Monoclonal Antibody

This antibody recognizes BPDE-I-DNA (PAH-DNA). Flow Cytometry: samples were fixed in 2% paraformaldehyde and permeabilized with 0.2% triton X-100/0.1% sodium citrate. Samples were treated with protK and RNase. To denature DNA, samples were incubated with 4n HCl. Samples were blocked with 5% normal serum before incubation with the monoclonal antibody. ELISA: plates were coated with 50 ng/well BPDE-DNA in 50mM Tris-buffer pH7.5 o/n at 4oC. Plates were blocked 1% FCS. DNA samples, 4 μg, were mixed with 5D11 and added to the well. Detection was performed using a Goat anti-Mouse IgG (AP) for 90 min. at 37oC. IHC (P): 5 μm sections were RNase and prot-K treated. DNA was denatured with 4N HCl and neutralized with 50mM Tris base. Sections were blocked with 1.5% normal horse serum prior to staining.

A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BPDE-DNA
Applications ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation
Classification Monoclonal
Clone 5D11
Concentration 0.1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.1% BSA and 0.02% sodium azide
Gene Alias Benzo[a]pyrenediol-epoxide; BPDE-DNA; BPDE-I-DNA; PAH-DNA
Host Species Mouse
Immunogen BPDE-I-DNA complexed with methylated BSA
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4° C
Product Type Antibody
Form Liquid
Isotype IgG2a
Target Molecule Chemical
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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