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Invitrogen™ BrdU Monoclonal Antibody (A9C6)
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Catalog No. FEMA550621
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100 μL
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FEMA550621 100 μL
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Catalog No. FEMA550621 Supplier Invitrogen™ Supplier No. MA550621
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Description

Mouse Monoclonal Antibody

Positive control: BrdU treated mouse embryo tissue, BrdU treated Hela, BrdU treated NIH/3T3, EdU treated mouse embryo tissue, EdU treated Hela, EdU treated NIH/3T3. Subcellular Location: Nucleus.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BrdU
Applications Dot Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone A9C6
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
Gene Alias 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Host Species Mouse
Immunogen BrdU-OVA.
Purification Method Protein G
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical, Mouse
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Mouse
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