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Invitrogen™ BrdU Polyclonal Antibody
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Catalog No. PIPA532256
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100 μL
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PIPA532256 100 μL
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Catalog No. PIPA532256 Supplier Invitrogen™ Supplier No. PA532256
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Description

Rabbit Polyclonal Antibody

Recommended positive controls: HeLa cells were treated with 100uM BrdU for 4hrs, U343 cells were treated with 10 μM BrdU for 18hrs. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
TRUSTED_SUSTAINABILITY

Specifications

Antigen BrdU
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 20% glycerol and 0.025% ProClin 300; pH 7
Gene Alias 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Host Species Rabbit
Immunogen BrdU.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Chemical
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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