Invitrogen™ eBioscience™ Brefeldin A Solution (1000X)

Catalog No.	Quantity
50-112-9757	1 mL

Catalog No. 50-112-9757 Supplier Invitrogen™ Supplier No. 00450651

Description

Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines.

• Brefeldin A is an inhibitor of intracellular protein transport
• Incubation of cells in culture with Brefeldin A leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER)
• Brefeldin A is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Monensin in their specific assay system

Specifications

• Quantity: 1 mL
• Product Line: eBioscience
• Physical Form: Liquid
• Product Type: Inhibitor

Frequently Asked Questions (FAQs)

What do Monensin and Brefeldin A do?

These are critical chemicals for inclusion in cell cultures during cell activation prior to analysis by flow cytometry for the presence of intracellular chemokines and cytokines. Monensin (Cat. No. 00-4505-51) and Brefeldin A (Cat. No. 00-4506-51) are protein transport inhibitors that block secretion of proteins by cells via the Golgi apparatus, thereby causing an accumulation of cytokines at the endoplasmic reticulum or Golgi. Cells are often incubated with either of these two chemicals during cell activation in order to promote the accumulation of detectable protein levels within the cell. These are often essential reagents that allow intracellular detection of proteins that would otherwise be too low in abundance to detect. Monensin is known to block the transport from the medial to the trans cisternae of the Golgi stack. Brefeldin A has been reported to block protein transport from the ER. Specific information about the use of Monensin and Brefeldin A can be found on their respective Technical Data Sheets.

For Research Use Only. Not for use in diagnostic procedures.