Invitrogen eBioscience Brefeldin A Solution, 1000x
Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines.
Manufacturer: Invitrogen 00450651
- Brefeldin A is an inhibitor of intracellular protein transport
- Incubation of cells in culture with Brefeldin A leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER)
- Brefeldin A is effective for enhanced detection of a majority of mouse and human intracellular cytokines; however, it is advised that the investigators evaluate the use and efficacy of this reagent as well as other protein transport inhibitors such as Monensin in their specific assay system
|Intracellular Staining Followed by Flow Cytometric Analysis|
|2° to 8°C|
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