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Thermo Scientific Chemicals Brilliant Blue G, ultrapure

Catalog No. AA4331818
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Quantity:
2 g
10 g
50 g

Protein staining following gel electrophoresis | CAS: 6104-58-1 | C47H48N3NaO7S2 | 854.025 g/mol

Brilliant Blue G is used in analytical biochemistry dye and as a P2X7 antagonist. It is also used in protein staining following gel electrophoresis. It is used to prepare the protein reagent for the determination of protein content of the collagenase enzyme isolated from fish waste. It may be employed as stain for the internal limiting membrane (ILM) for the macular hole (MH) and epiretinal membrane (ERM) surgery. It is useful for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualized during staining. It has been used in the Bradford dye-binding protein assay. A mechanism for dye binding to protein has been proposed, based on measuring Coomassie Brilliant Blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant Blue G has been used for determining critical micelle concentration of detergents.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 6104-58-1
Molecular Formula C47H48N3NaO7S2
Molecular Weight (g/mol) 854.025
MDL Number MFCD00078482
InChI Key RWVGQQGBQSJDQV-UHFFFAOYSA-M
Synonym Coomassie Brilliant Blue G 250; C.I. 42655
PubChem CID 6328534
IUPAC Name sodium;3-[[4-[(Z)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-N-ethyl-3-methylanilino]methyl]benzenesulfonate
SMILES CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC(=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3C)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C.[Na+]

Specifications

Solubility Information Soluble in water (50mg/ml) and ethanol (40mg/ml).
Formula Weight 854.04
Quantity 50 g
Physical Form Powder
Chemical Name or Material Brilliant Blue G, Ultrapure
Hazard Category H500
EINECSNumber 228-058-4
TSCA Yes
Recommended Storage Ambient temperatures

RUO – Research Use Only

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