Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Catalog No.	Quantity
PI21586	1 g
PIA39266	10 x 2 mg
PI21580	50 mg

Catalog No. PI21586 Supplier Thermo Scientific™ Supplier No. 21586

Description

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Highlights:

• Crosslink cell-surface proteins before cell lysis and immunoprecipitation
• Identify receptor-ligand interactions by chemical crosslinking
• “Fix” protein interactions to allow identification of weak or transient protein interactions
• Identify near-neighbor protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Immobilize proteins onto amine-coated surfaces

Specifications

• Chemical Reactivity: Amine-Amine
• Cleavable: No
• Molecular Weight (g/mol): 572.43
• PEGylated: No
• Spacer Arm Length: 11.4 Å
• Content And Storage: Upon receipt store desiccated at 4°C.
• Cell Permeability: No
• Shipping Condition: Ambient
• Product Line: Pierce
• Labeling Method: Chemical Labeling
• Crosslinker Type: Homobifunctional
• Reactive Moiety: Sulfo-NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Powder
• Quantity: 1 g
• Solubility: Water
• Format: Standard, Single-use, Premium-grade
• Water Soluble: Yes
• Product Type: Crosslinker

Frequently Asked Questions (FAQs)

What is the difference between DSS and BS3 crosslinkers?

BS3 is the water-soluble analogue of DSS (i.e., DSS is the membrane-permeable analog of BS3). DSS is water-insoluble and membrane-permeable, so it can be used for both intracellular and cell surface crosslinking. BS3 is water-soluble and membrane-impermeable, so it can be used for cell-surface crosslinking.

What is the protocol for crosslinking IgG to Dynabeads from the Dynabeads Protein G Immunoprecipitation Kit (Cat. No. 10007D) using DSS (disuccinimidyl suberate)?

For crosslinking the antibody to Protein G Dynabeads, please refer to the crosslinking protocol with BS3 (bis(sulfosuccinimidyl)suberate) or Sulpha-DSS, which is an analogue of DSS. The only difference between these two molecules is that DSS is water insoluble and needs to be solubilized in DMSO before use. You can find the protocol at the following link.

Can you provide the shelf-life for BS3 (bis(sulfosuccinimidyl)suberate)?

BS3 (bis(sulfosuccinimidyl)suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

What is the difference between a homo- and a hetero- bifunctional cross-linker?

A homobifunctional cross-linker has the same reactive group on both ends, for example two amine-reactive groups. It is used in a one-step conjugation where the two molecules are mixed prior to the addition of the cross-linker. This leads to less efficient cross-linking because polymers can easily be created. A heterobifunctional cross-linker has two different reactive groups, for example an amine-reactive group on one end and a sulhydryl reactive group on the other. These can be used in two-step conjugations by reacting the fIrst end with the molecule containing its reactive group, and then adding the molecule that is reactive towards the other end. This leads to much more efficient cross-linking because polymerization does not occur.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.