Invitrogen™ c-Fos Monoclonal Antibody (2G9C3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA121190

Catalog No. PIMA121190

Description

This antibody does not cross-react with the 39 kDa jun protein. By Western blot, this antibody detects ~55 & 62 kDa bands representing c-fos and v-fos, respectively. MA1-21190 is not suitable for IHC applications on frozen or paraffin tissues. Positive control of HeLa Cells suggested.

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

Specifications

• Antigen: c-Fos
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: 0.05M sodium phosphate with 0.2% gelatin and 0.1% sodium azide; pH 7.5
• Gene Accession No.: P01100, P01101, P12841
• Gene Symbols: FOS
• Immunogen: Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot, Immunocytochemistry
• Clone: 2G9C3
• Conjugate: Unconjugated
• Gene: FOS
• Gene Alias: activator protein 1; AP-1; cellular oncogene c-fos; cellular oncogene fos; cFos; C-FOS; c-fos oncogene; c-fos protooncogene protein; D12Rfj1; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FBJ murine osteosarcoma viral oncogene homolog B; FBJ osteosarcoma oncogene; FBJ osteosarcoma oncogene B; FBJ osteosarcoma viral oncogene isoform deltaFosb-2; FOS; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; Fosb; FosB proto-oncogene, AP-1 transcription factor subunit; fra-2; G0/G1 switch regulatory protein 7; G0S7; I79_009428; p55; protein fosB; proto-oncogene cFOS; proto-oncogene c-Fos; proto-oncogene protein c-fos; v-fos FBJ murine osteosarcoma viral oncogene homolog
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 14281, 2353, 314322
• Content And Storage: 4°C, do not freeze
• Form: Liquid