Invitrogen™ c-Rel Monoclonal Antibody (1RELAH5), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-611-180	25 μg

Catalog No. 50-611-180 Supplier Invitrogen™ Supplier No. 50611180

Description

Rat Monoclonal Antibody

The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells. Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor 660 is a replacement for Alexa Fluor 647. eFluor 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser:...

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

Specifications

• Antigen: c-Rel
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 1RELAH5
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 660
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: REL
• Gene Accession No.: P15307
• Gene Alias: BCD541; C-Rel; C-Rel protein; C-Rel proto-oncogene protein; gemin 1; gemin-1; LOW QUALITY PROTEIN: proto-oncogene c-Rel; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; REL; REL proto-oncogene, NF-kB subunit; reticuloendotheliosis oncogene; SMA; SMA1; SMA2; SMA3; SMA4; SMN; SMN2; SMNT; v-rel avian reticuloendotheliosis viral oncogene homolog; v-rel reticuloendotheliosis viral oncogene homolog
• Gene Symbols: REL
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19696
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov