Invitrogen™ Calbindin D28K Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1931

Catalog No. PA1931

Description

PA1-931 detects calbindin D-28k protein in rat samples. PA1-931 has successfully been used in Western blot , ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 28 kDa protein representing calbindin D from rat cerebellum and rat kidney extract. Immunohistochemical staining on paraffin sections demonstrates that calbindin D is localized to the cytoplasm in epithelial cells of distal tubules in human kidney cortex after staining with PA1-931. The PA1-931 immunizing protein corresponds to the purified 28 kDa calbindin-D protein from rat kidney. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

Specifications

• Antigen: Calbindin D28K
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CALB1
• Gene Alias: Brain-2; CAB27; CaBP28K; Calb; CALB1; Calb-1; Calbindin; calbindin 1; calbindin 1, (28kD); calbindin 1, 28 kD; calbindin 1, 28kDa; calbindin D28; calbindin-28K; calbindin-D (28k); CalbindinD28K; CB; cerebellar Ca-binding protein spot 35 protein; cerebellar Ca-binding protein, spot 35 protein; D-28K; PCD-29; RTVL-H protein; spot 35 protein; Vitamin D-dependent calcium-binding protein, avian-type
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12307, 793, 83839
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 15mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P05937, P07171, P12658
• Gene Symbols: CALB1
• Immunogen: 28 kDa calbindin-D protein purified from rat kidney.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG