Invitrogen™ Calnexin Monoclonal Antibody (AF18), Alexa Fluor™ 488

Catalog No.	Quantity
PIMA3027A48	50 μL

Catalog No. PIMA3027A48 Supplier Invitrogen™ Supplier No. MA3027A488

Description

Mouse Monoclonal Antibody

MA3027A488 detects calnexin from human and mouse tissues. The MA3027A488 antigen is Focus human hepatoma cell lysate.

Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).

Specifications

• Antigen: Calnexin
• Applications: Immunocytochemistry
• Classification: Monoclonal
• Clone: AF18
• Concentration: 1 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.4
• Gene: CANX
• Gene Accession No.: P27824, P35564
• Gene Alias: 1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor
• Gene Symbols: CANX
• Host Species: Mouse
• Immunogen: Focus human hepatoma cell lysate.
• Purification Method: Protein A
• Quantity: 50 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12330, 821
• Target Species: Human, Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1