Cannabinoid Receptor 1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA1745

Catalog No. PIPA1745

Description

PA1-745 detects cannabinoid receptor 1 (CB1) from human, rat, monkey, mouse, chicken, salamander and goldfish tissues. This antibody detects a few non-specific bands in NIH-3T3 and HT-29 cell lysates. PA1-745 has been successfully used in Western blot, FACS, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects a ∽60 kDa protein representing CB1 and less intense bands of ∽23 kDa, ∽72 kDa, and ∽180 kDa from rat brain homogenate. Immunocytochemical staining of CB1 in living or fixed cultured hippocampal neurons with PA1-745 results in intense, punctate staining along processes, with little staining over cell bodies. PA1-745 antigen is a fusion protein containing the first 77 amino acid residues of the rat CB1 receptor.

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

Specifications

• Antigen: Cannabinoid Receptor 1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CNR1
• Gene Alias: brain-type cannabinoid receptor, CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB1, CB1 cannabinoid receptor, CB1 receptor, CB1A, CB1K5, CB1R, CB-R, central cannabinoid receptor, CNR, Cnr1, HGNC:2159, OTTHUMP00000016839, RP1-23D17.1, seven transmembrane domains, G-protein-coupled receptor, Skr6, SKR6R, striatal cannabinoid receptor type 1 protein
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1268, 12801, 25248, 428633
• Content And Storage: -20°, C, Avoid Freeze/Thaw Cycles
• Isotype: IgG

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1.0 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P20272, P21554, P47746
• Gene Symbols: CNR1
• Immunogen: A fusion protein containing the first 77 amino acid residues from rat CB1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Amphibian, Chicken, Fish, Human, Mouse
• Form: Liquid