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Thermo Scientific™ CaptureSelect™ AAT-XL Affinity Matrix
Description
CaptureSelect AAT-XL Affinity Matrix has been specifically designed for the purification of native human alpha-1 antitrypsin (AAT) from complex sources like plasma/serum and recombinant human AAT from cell culture supernatants. This affinity matrix offers mild elution conditions at pH 6. The high selectivity and yield obtained using CaptureSelect AAT-XL Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in a single step.
Features of this affinity matrix include:
- Highly selective for native and recombinant human AAT
- High recovery and yield obtained in a single purification step
- Mild elution at pH 6
- Excellent scalability
- Non-animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics:
- Matrix: agarose-based, epoxide-activated
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect AAT-XL affinity ligand
- Ligand coupling method: epoxide coupling
- Binding capacity: 15 mg AAT/mL resin
- Elution conditions: 20 mM Tris, 2.0 M MgCl2, pH 6.0
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Binding Capacity | 15 mg AAT/mL resin |
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide-activated |
| Particle Size | 65 ±10 μm |
| Form | Resin Slurry |
| Product Line | CaptureSelect |
| Quantity | 5 mL |
| Type | Affinity Matrix |
| Ligand Type | AAT-XL affinity ligand |
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