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Thermo Scientific™ CaptureSelect™ C-tagXL Affinity Matrix
Description
CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. It purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. C-tagXL Affinity Matrix recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.
Features of C-tagXL Affinity Matrix include:
- Mild elution, making it suitable for pH-sensitive proteins
- Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
- Excellent scalability
- Non-animal-derived
CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics:
- Matrix: agarose-based, epoxide-activated
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect C-tagXL affinity ligand
- Ligand coupling method: epoxide coupling of the ligand
- Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time
- Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl2 pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A.
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20%(v/v) ethanol
Specifications
Specifications
| Binding Capacity | 400 nmol/mL |
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide-activated |
| Content And Storage | Store at 2°C to 8°C. |
| Shipping Condition | Room Temperature |
| Particle Size | 65 ±10 μm |
| Form | Liquid Suspension |
| Product Line | CaptureSelect |
| Quantity | 50 mL |
| Type | Affinity Matrix |
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