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Thermo Scientific™ CaptureSelect™ Factor X Affinity Matrix
Description
CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.
Features of the CaptureSelect Factor X Affinity Matrix include:
- Highly selective for human Factor X with no affinity for other human plasma proteins
- Binds both Factor X and Factor Xa in the presence of calcium
- Enables efficient purification of Factor X with high yields and retained activity
- Mild elution at neutral pH using EDTA
- Offers excellent scalability
- Non-animal-derived
Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
- Matrix: agarose-based
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect Factor X affinity ligand
- Ligand coupling method: epoxide
- Binding capacity: >10 mg/mL resin
- Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7
- Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Binding Capacity | >10 mg/mL |
| Flow Rate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based |
| Particle Size | 65 ±10 μm |
| Form | Resin Slurry |
| Product Line | CaptureSelect™ |
| Quantity | 250 mL |
| Type | Affinity Matrix |
| Ligand Type | Factor X affinity ligand |
| Stationary Phase | Factor X |
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