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Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate
Description
CaptureSelect HRP Anti-AAVX Conjugate includes a 14-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of multiple AAV serotypes. The antibody fragment binds with high affinity to AAV particles and demonstrates good binding reactivity towards a variety of AAV serotypes, including AAV1 to AAV8, and AAVrh10. The antibody fragment is chemically conjugated to horseradish peroxidase (HRP) via a spacer of appropriate length to maintain full binding capacity of the antibody fragment.
CaptureSelect HRP Anti-AAVX Conjugate can be combined with CaptureSelect Biotin Anti-AAVX Conjugate (Cat. No. 7103522100) in enzyme linked immunoassays to quantify the total number of AAV capsids. Sandwich ELISAs can be designed using the biotin anti-AAVX conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAVX conjugate is then used for detection of the bound AAV capsids. The unique binding properties of this pair of anti-AAVX conjugates can circumvent the need for incorporation of different AAV serotype-specific antibodies for AAV assay development.
For assay development to measure AAV9 total capsids, we recommend CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) and CaptureSelect HRP Anti-AAV9 Conjugate (Cat. No. 7303332100).
Product details
- Format type: HRP, liquid
- Buffer: PBS, 50% (v/v) glycerol
- Storage: in freezer (-5 to -30°C)
- Concentration: 0.5 mg/mL
- Volume: 0.1 mL
- Recommended working dilution for sandwich ELISA: 1: 5000 to 1: 10,000 (the optimal working dilution should be determined empirically for each application)
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Specifications
Specifications
| Concentration | 0.5 mg/mL |
| Formulation | Liquid, in PBS (7.4), 50% glycerol |
| Content And Storage | Store in freezer (-5°C to -30°C). |
| Form | Liquid |
| Product Line | CaptureSelect™ |
| Quantity | 100 μL |
| For Use With (Application) | Quantitation of multiple AAV capsids |
| Type | Conjugate |
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