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  6. Thermo Scientific™ CaptureSelect™ Human Fab-kappa Kinetics Biotin Conjugate

Thermo Scientific™ CaptureSelect™ Human Fab-kappa Kinetics Biotin Conjugate

Catalog No. 7103302100
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Quantity:
100 μg
500 μg

Description

Thermo Scientific™ CaptureSelect™ Human Fab-kappa Kinetics Biotin Conjugate is a bi-functional ligand construct consisting of 2 different 13 kDa recombinant single domain antibody fragments (affinity ligands).

CaptureSelect Human Fab-kappa Kinetics Biotin Conjugate is a bi-functional ligand construct consisting of 2 different 13 kDa recombinant single domain antibody fragments (affinity ligands).

The CaptureSelect anti-LC-kappa (Hu) ligand that specifically binds to the constant domain of human kappa light chains is genetically fused to the CaptureSelect anti-IgG-CH1 ligand that specifically binds to the CH1 domain of all four human IgG subclasses. The bi-functional affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect Human Fab-kappa Kinetics Biotin Conjugate allows you to:

  • Screen Fab-antigen interactions—the Human Fab-kappa Kinetics Biotin Conjugate allows high affinity capture of human IgG Fab fragments that possess a kappa light chain. Extreme low off-rates [k(diss)] are achieved through avidity by targeting the CL-kappa domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Applications for CaptureSelect Human Fab-kappa Kinetics Biotin Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Specifications

Content And Storage Store in freezer (-5°C to -30°C).
Form Liquid
Product Line CaptureSelect™
Quantity 100 μg
Type Conjugate
Label or Dye Biotin & Other Haptens
Ligand Type Antibody
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