Invitrogen™ Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA126180

Catalog No. MA126180

Description

This antibody is myosin alpha and beta heavy chain specific. Affinity constants: Human ventricular myosin heavy chains - 3.33 x 108M-1; Human atrial myosin heavy chains - 1.48 x 108M-1; Human skeletal muscle myosin - 1.06 x 108M-1; Canine cardiac myosin heavy chains - 2.06 x 108M-1. This antibody cross-reacts with heavy chains of human myosin isolated from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells. Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.

Cardiac Myosin Heavy Chain (MHC) is a crucial component of the contractile machinery in heart muscle cells. It primarily forms part of the thick filament in sarcomeres, the fundamental units of muscle contraction. The cardiac myosin heavy chain comes in two isoforms, alpha-MHC and beta-MHC, which are encoded by the MYH6 and MYH7 genes, respectively. The alpha-MHC isoform is predominant in the atria and is characterized by faster ATPase activity and contraction speed, whereas the beta-MHC isoform is predominant in the ventricles and is associated with greater endurance. Each myosin molecule consists of two heavy chains, two essential light chains (ELC), and two regulatory light chains (RLC). The heavy chains have distinct functional regions: the globular head, which binds to actin and hydrolyzes ATP to produce force, and the helical tail, which organizes into the thick filament backbone. Mutations in cardiac MHC can lead to various cardiomyopathies, including hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), both of which significantly affect the heart's functionality. These disorders underscore the importance of MHC in maintaining normal cardiac physiology and highlight its complex regulation and mechanotransduction capabilities.

Specifications

• Antigen: Cardiac Myosin Heavy Chain
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: A2AQP0, A7E2Y1, P02563, P02564, P12883, P13533, Q02566, Q91Z83, Q9BE39
• Gene Symbols: MYH6, Myh7, Myh7b
• Immunogen: Human ventricular myosin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Clone: 3-48
• Conjugate: Unconjugated
• Gene: Myh7b
• Gene Alias: A830009F23Rik; AA517445; alpha cardiac MHC; alpha myosin; alpha-cardiac myosin heavy chain; alpha-MHC; alpha-myosin; alpha-myosin heavy chain; alpha-myosin heavy chain subfragment 2 exon; antigen MLAA-21; ASD3; beta cardiac myosin heavy chain; beta myosin heavy chain; beta-MHC; beta-myosin; beta-myosin heavy chain; B-MHC; cardiac muscle myosin heavy chain 7 beta; cardiac myosin heavy chain alpha isoform; cardiac myosin heavy chain beta isoform; cardiomyopathy, hypertrophic 1; CMD1EE; CMD1S; CMH1; CMH14; KIAA1512; LOC100736765; LOW QUALITY PROTEIN: myosin-6; LOW QUALITY PROTEIN: myosin-7B; MHC14; MPD1; Myh14; MYH6; MYH7; Myh7b; MYH-beta/slow; MYHC; Myhca; Myhc-a; MyHC-alpha; MYHCB; Myhc-b; MyHC-beta; MyHC-I; MyHC-slow; myopathy, distal 1; myosin alpha heavy chain; Myosin cardiac muscle beta chain; myosin heavy chain; myosin heavy chain 6; Myosin heavy chain 7; myosin heavy chain 7B; myosin heavy chain 7B, cardiac muscle beta isoform; myosin heavy chain polypeptide 6 cardiac muscle adult; myosin heavy chain polypeptide 7 cardiac muscle fetal; myosin heavy chain slow; myosin heavy chain slow isoform; myosin heavy chain slow type 1 (beta cardiac); myosin heavy chain, cardiac; myosin heavy chain, cardiac muscle alpha isoform; Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha); myosin heavy chain, cardiac muscle beta isoform; myosin heavy chain, cardiac muscle, adult; myosin heavy chain, cardiac muscle, fetal; myosin heavy chain, polypeptide 6, cardiac muscle, alpha; myosin heavy chain, polypeptide 7; myosin, heavy chain 6, cardiac muscle, alpha; myosin, heavy chain 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy chain 7B, cardiac muscle, beta; myosin, heavy polypeptide 6, cardiac muscle, alpha; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin, heavy polypeptide 7, cardiac muscle, beta; myosin, heavy polypeptide 7B, cardiac muscle, beta; myosin-6; Myosin-7; myosin-7B; rhabdomyosarcoma antigen MU-RMS-40.7A; slow A MYH14; slow myosin heavy chain; slow myosin heavy chain-beta; slow type myosin heavy chain; SPMD; SPMM; SSS3; type slow/beta myosin heavy chain; U937-associated antigen; Unknown (protein for IMAGE:7985957)
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100296004, 140781, 17888, 282714, 29556, 29557, 311570, 4624, 4625, 521764, 57644, 668940
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov