Invitrogen™ Cas12j Monoclonal Antibody (5F95)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547448

Catalog No. PIMA547448

Description

Cas12j Monoclonal Antibody for Western Blot, ICC/IF

There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. The lab of Nobel prize winner Jennifer Doudna characterized these Cas enzymes and showed that they could be used to perform CRISPR, but had the significant advantage that they were much smaller in molecular size than other Cas family enzymes such as the widely used Cas9 enzymes from S. pyogenes and S. aureus. One of these enzymes is referred to as Cas theta and also known as Cas-12j. The use of such smaller Cas family enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.

Specifications

• Antigen: Cas12j
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 5mM sodium azide
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot, Immunocytochemistry
• Clone: 5F95
• Conjugate: Unconjugated
• Gene Alias: Cas theta
• Immunogen: Recombinant Cas theta/Cas-12j expressed in and purified from E. coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Chemical

Safety and Handling

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