Invitrogen™ Caspase 3 p17 Monoclonal Antibody (31A1067)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547699

Catalog No. PIMA547699

Description

The antibody detects pro Caspase-3 at 32 kDa* and the large subunit of the cleaved form of Caspase-3 at 14-21 kDa. The antibody can be used for western blot, immunohistochemistry and immuno-electron microscopy.

CASP3 (Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Tiso et al. (1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al. (1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al. (1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.

Specifications

• Antigen: Caspase 3 p17
• Classification: Monoclonal
• Concentration: 0.4 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P42574, P55213, P70677
• Gene Symbols: CASP3
• Immunogen: Clone 31A1067 was developed against full-length recombinant human Caspase-3. The antibody recognizes an epitope in the large domain subunit (p17) of Caspase-3, and it will recognize pro Caspase-3 and the p17 cleavage fragment.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: 31A1067
• Conjugate: Unconjugated
• Gene: CASP3
• Gene Alias: A830040C14Rik; AC-3; Apopain; Casp3; CASP-3; caspase 3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; CC3; Cleaved Caspase 3; Cpp32; CPP-32; CPP32beta; cysteine protease CPP32; IRP; LICE; mldy; protein Yama; SCA-1; SREBP cleavage activity 1; Yama; yama protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12367, 25402, 836
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid