Invitrogen™ CBF beta Polyclonal Antibody

Catalog No.	Quantity
01-670-101	100 μg

Catalog No. 01-670-101 Supplier Invitrogen™ Supplier No. PA1317

Description

Rabbit Polyclonal Antibody

PA1-317 detects CBF-beta in human samples. PA1-317 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~22 kDa protein representing CBF-beta in HeLa whole cell lysates. The PA1-317 immunogen is a synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta. This sequence is conserved in mouse. PA1-317 immunizing peptide (Cat. # PEP-282) is available for use in neutralization and control experiments.

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CBF beta
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: CBFB
• Gene Accession No.: Q13951
• Gene Alias: AI893578; CBFB; CBF-beta; core binding factor beta; core-binding factor subunit beta; core-binding factor, beta subunit; PEA2; PEA2-beta; Pebp2; PEBP2B; PEBP2-beta; Pebpb2; polyomavirus enhancer binding protein 2, beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; SL3/AKV core-binding factor beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta
• Gene Symbols: CBFB
• Host Species: Rabbit
• Immunogen: Synthetic peptide corresponding to residues M(1) P R V V P D Q R S K F E N E E F F R K(20) of human CBF beta.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 865
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov