Novus Biologicals™ CCL14/HCC-1/HCC-3 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human CCL14/HCC-1/HCC-3.

Specifications

• Assay Range: 18.75 pg/mL
• Assay Sensitivity: 18.75-1200 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human CCL14/HCC-1/HCC-3.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: C-C motif chemokine 14, chemokine (C-C motif) ligand 14, Chemokine CC-1/CC-3, chemokine CC-3, CKB1, FLJ16015, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, hemofiltrate CC chemokine 1, MCIF, member 14, NCC2CC-1, NCC-2CKb1, new CC chemokine 2, SCYA14CC-3, Small-inducible cytokine A14
• Gene ID (Entrez): 6358
• Gene Symbol: CCL14
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human CCL14/HCC-1/HCC-3 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Human CCL14/HCC-1/HCC-3 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 55 ng of recombinant Human CCL14/HCC-1/HCC-3.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Biologically Active Proteins, Cell Cycle and Replication, Chemokines and Cytokines
• Storage Requirements: Storage is content dependent.
• Target: CCL14/HCC-1/HCC-3
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.