Novus Biologicals™ CCL2/JE/MCP-1 Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse CCL2/JE/MCP-1.

Specifications

• Assay Range: 7 pg/mL
• Assay Sensitivity: 7-450 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse CCL2/JE/MCP-1.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, Mcp1, Mcp-1, SCYA2, SMC-CF
• Gene ID (Entrez): 6347
• Gene Symbol: CCL2
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of rabbit anti-CCL2/JE/MCP-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of rabbit anti-Mouse CCL2/JE/MCP-1 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 28 ng of recombinant Mouse CCL2/JE/MCP-1.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: CCL2/JE/MCP-1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 450 pg/mL is recommended.