Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF. Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5 antibody, cat. 16-7096, in functional assays.). Applications Tested: Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombit mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombit human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
|Human, Mouse, Rat|
|ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok