Invitrogen™ CCL7 (MCP-3) Monoclonal Antibody (OLGMASCE), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-112-4432	25 Tests

Catalog No. 50-112-4432 Supplier Invitrogen™ Supplier No. 50707741

Description

Mouse Monoclonal Antibody

Description: This OLGMASCE monoclonal antibody reacts with human CCL7. CCL7, also known as MCP-3 (Monocyte Chemotactic Protein 3), is a member of the CC- subfamily of chemokines and is most closely related to CCL2 (MCP-1) and CCL8 (MCP-2). They are secreted by a variety of cell types in response to inflammatory stimuli and play critical roles in the recruitment of leukocytes to areas of inflammation. While all three MCP proteins are potent chemoattractants of monocytes and T cells, CCL7 appears to have the broadest range of activity, as it has also been demonstrated to attract activated NK cells, eosinophils, basophils, and neutrophils. CCL7 signals via the g protein-coupled receptors CCR1 and CCR2, both of which are shared with other CC-chemokines. Applications Reported: This OLGMASCE antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This OLGMASCE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor...

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

Specifications

• Antigen: CCL7 (MCP-3)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: OLGMASCE
• Concentration: 5 μL/Test
• Conjugate: eFluor 660
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: CCL7
• Gene Accession No.: P80098
• Gene Alias: C-C motif chemokine 7; C-C motif chemokine ligand 7; CCL7; chemokine (C-C motif) ligand 7; chemotactic protein-3; Fic; H-MCP-3; Intercrine/chemokine MARC; marc; Mcp3; MCP-3; MGC138463; MGC138465; Monocyte chemoattractant protein 3; monocyte chemotactic protein 3; NC28; Protein FIC; RANTES/sis homolog; RP23-350G1.4; SCYA6; Scya7; small inducible cytokine A7; small inducible cytokine A7 (monocyte chemotactic protein 3); small-inducible cytokine A7
• Gene Symbols: CCL7
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 6354
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.