Invitrogen™ CCL8 (MCP-2) Monoclonal Antibody (DWZEE), eFluor™ 660, eBioscience™

Catalog No.	Quantity
50-274-1165	100 Tests

Catalog No. 50-274-1165 Supplier Invitrogen™ Supplier No. 50978942

Description

Mouse Monoclonal Antibody

Description: This DWZEE monoclonal antibody reacts with human CCL8. CCL8, also known as MCP-2 (Monocyte Chemotactic Protein 2), is a member of the CC- subfamily of chemokines and is most closely related to CCL2 (MCP-1) and CCL7 (MCP-3). They are secreted by a variety of cell types in response to inflammatory stimuli and play critical roles in the recruitment of leukocytes to areas of inflammation. While all three MCP proteins are potent chemoattractants of monocytes and T cells, CCL7 appears to have the broadest range of activity, as it has also been demonstrated to attract activated NK cells, eosinophils, basophils, and neutrophils. CCL8 has also been demonstrated to attract NK cells, eosinophils, and basophils, but requires higher concentrations to do so. CCL8 signals via the g protein-coupled receptor CCR5, which is shared with other CC-chemokines. CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization. Applications Reported: This DWZEE antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This DWZEE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood monocytes. This can be used at 5 μL (0.125...

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.

Specifications

• Antigen: CCL8 (MCP-2)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: DWZEE
• Concentration: 5 μL/Test
• Conjugate: eFluor 660
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: CCL8
• Gene Accession No.: P80075
• Gene Alias: 1810063B20Rik; AB023418; C-C motif chemokine 8; C-C motif chemokine ligand 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; MCP-2(6-76); monocyte chemoattractant protein 2; monocyte chemoattractant protein-2; monocyte chemotactic protein 2; SCYA10; SCYA8; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); small-inducible cytokine A8
• Gene Symbols: CCL8
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 6355
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

Is the eFluor 660 fluorochrome compatible with Anti-Cy5/Alexa Fluor 647 beads?

Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.