Invitrogen™ CCR7 Monoclonal Antibody (4B12)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA1163

Catalog No. PIMA1163

Description

MA1-163 has been validated for flow cytometry on primary mouse splenocytes. Optimal staining is observed when incubating the cells with antibody at 37C for at least 30 minutes. MA1-163 has been validated for Western blot on Jurkat cell lysates.

CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation.

Specifications

• Antigen: CCR7
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: P32248, P47774
• Gene Symbols: Ccr7
• Immunogen: Rat basophilic leukaemia (RBL)-2H3 transfectants expressing mouse CCR7.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Western Blot
• Clone: 4B12
• Conjugate: Unconjugated
• Gene: Ccr7
• Gene Alias: BLR2; Bukitt's lymphoma receptor 2; CC chemokine receptor 7; C-C chemokine receptor type 7; C-C CKR-7; C-C motif chemokine receptor 7; CC-CKR-7; CCR7; CCR-7; CD197; CDw197; chemokine (C-C motif) receptor 7; chemokine (C-C) receptor 7; Cmkbr7; EBI1; Ebi1h; EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 1236, 12775
• Content And Storage: -20°C
• Form: Liquid