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Gibco™ CD OptiCHO™ AGT™ Medium
Description
CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco™ CD CHO's legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit (Cat. No. A1024101) feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.
When to Use CD OptiCHO™ Medium
- You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
- Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
- Your CHO cell line is 'finicky,' i.e. hard to grow or adapt
When to Consider Another Gibco™ Medium
Consider CD FortiCHO™ medium (Cat. No. A1148301) when:
- The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
- Maximum batch culture cell densities and protein titers are needed
- You have time to adapt cells into a new medium in an effort to maximize titers
- Cell health at harvest is a priority for downstream processing
Consider CD CHO medium (Cat. No. 10743029) when:
- Your GS CHO cells are recommended to be grown in CD CHO medium
- Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium (Cat. No. 12610010) when:
- You use parental DG44 or DXB11 CHO cells (dhfr-)
Liquid Media Formulations
Gibco™ AGT™ media are also available in a liquid format (Cat. No. 12681011) that offers the same performance and formulation as the AGT™ media format. Gibco™ liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.
Design Your Own Media Specific to Your Needs
Whether it's a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Specifications
Specifications
| Cell Line | CHO |
| Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
| Form | Powder (AGT) |
| Product Type | CD OptiCHO AGT Medium |
| Serum Level | Serum-free |
| Sterility | Non-sterile |
| With Additives | High Glucose, Sodium Pyruvate, Sodium Bicarbonate |
| Without Additives | No Glutamine, No Phenol Red |
| Product Line | AGT, Gibco |
| Quantity | 1 L |
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Frequently Asked Questions (FAQs)
Complete formulations
- Homogeneous distribution of minute components
- Complete complex formulations in a dry format, including serum-free, protein-free, and chemically defined nutrient media
- Pre-adjusted to the appropriate pH and osmolality
- Require only standard supplementation with L-glutamine for L-glutamine dependent systems
- Can be customized for modified catalog and customers' proprietary formulations
Granular format
- Dissolves instantly for faster media preparation times
- Dust-free granules reduce mess and environmental contamination
- Performance equivalent to liquid media
- Workflow improvement
- Helps reduce total cycle costs due to lower number of components, decreasing time involved in raw material planning, procurement, and testing
- Faster and easier media preparation, with less potential for error
- Easy to scale up from research through production, in batch sizes from 2 kg to 6,000 kg
- Available in various packaging options
Granulated cell culture media is an easy-to-use, granular form of dry media that is produced by applying fluid bed granulation technology to cell culture nutrient media manufacturing. The patented process allows production of complete formulations of a variety of serum-free, protein-free, and chemically defined media in a dry format.
AGT offers the benefits of liquid media without the cost, storage, and transportation issues. It is a complete medium that is pH and osmolality pre-adjusted. The granules dissolve instantly for faster media preparation time than conventional dry powder media. AGT media can therefore help reduce total cycle costs, decreasing time involved in raw material planning, procurement, and testing as well as media preparation. These benefits are all realized while maintaining comparable cell growth to liquid media.
For Research Use Only. Not for use in diagnostic procedures.
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