Invitrogen™ CD101 Monoclonal Antibody (Moushi101), Alexa Fluor™ 700, eBioscience™

Catalog No.	Quantity
56-101-182	100 μg

Catalog No. 56-101-182 Supplier Invitrogen™ Supplier No. 56101182

Description

Rat Monoclonal Antibody

Description: The monoclonal antibody Moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains). CD101 is a transmembrane glycoprotein containing 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation. CD101 is expressed on monocytes, granulocytes, dendritic cells, Langerhans cells and activated T cells. In addition, CD101 is found on a subset of CD25+Foxp3+ T regulatory cells that showed higher suppressive properties in vitro and in vivo. Applications Reported: This Moushi101 antibody has been reported for use in flow cytometric analysis. Applications Tested: This Moushi101 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

CD101, also known as the V7 antigen, is a transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is characterized by 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites. CD101 forms a disulfide-linked homodimer, although its precise function remains unknown. Expression of CD101 is observed on monocytes, granulocytes, dendritic cells, Langerhans cells, and activated T cells. It is also found on a subset of CD25+Foxp3+ regulatory T cells, which exhibit higher suppressive properties in vitro and in vivo. CD101 plays a role in modulating immune responses. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation, while CD101+ CD28+ T cells are highly responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can enhance proliferation, suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. In mice, CD101 is expressed on a subpopulation of regulatory T cells (CD4+, CD25+, Foxp3+) with high suppressor activity. In humans, CD101 expression on peripheral blood mononuclear cells (PBMCs) stains approximately 30% of Foxp3+ cells, although studies have not confirmed higher suppressor activity in the human CD101+ population.

Specifications

• Antigen: CD101
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: Moushi101
• Concentration: 0.2 mg/mL
• Conjugate: Alexa Fluor 700
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: CD101
• Gene Accession No.: A8E0Y8
• Gene Alias: Cd101; CD101 antigen; CD101 molecule; cell surface glycoprotein V7; EWI immunoglobulin subfamily member EWI-101; EWI101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; Gm1016; Gm734; IGSF2; Immunoglobulin superfamily member 2; immunoglobulin superfamily, member 2; leukocyte surface protein; RP11-27K13.2; V7
• Gene Symbols: CD101
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 630146
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Safety and Handling

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