CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 14105182

Catalog No. 50-127-98

Description

Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis. Applications Reported: The MJ7/18 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. Applications Tested: The MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells or mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Specifications

• Antigen: CD105 (Endoglin)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q63961
• Gene Symbols: ENG
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13805
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: MJ7/18
• Conjugate: Unconjugated
• Gene: ENG
• Gene Alias: AI528660; AI662476; CD105; CD105 antigen; cell surface MJ7/18 antigen; Edg; END; Endo; Endoglin; endoglin (Osler-Rendu-Weber syndrome 1); Eng; HHT1; ORW; ORW1; S-endoglin; transmembrane glycoprotein
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.