Description: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease. Applications Reported: This eBioH4B4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioH4B4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins." This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm).CD107b (LAMP-2) Monoclonal antibody specifically detects CD107b (LAMP-2) in Human samples. It is validated for Flow Cytometry.
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