CD117 (c-Kit) Mouse anti-Human, Clone: YB5.B8, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-128-34	Invitrogen 14117980	25 μg
50-128-35	Invitrogen 14117982	100 μg

Description

Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells. Applications Reported: YB5.B8 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1&2 signaling pathway.

Specifications

• Antigen: CD117 (c-kit)
• Target Species: Human
• Isotype: IgG1 κ
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

• Clone: YB5.B8
• Conjugate: Unconjugated
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Form: Liquid
• Product Type: Antibody
• Content And Storage: 4° C

For Research Use Only.