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CD117 (c-Kit) Rat anti-Mouse, Porcine, PE-Cyanine5, Clone: 2B8, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$152.00 - $358.00

Specifications

Antigen CD117 (c-Kit)
Clone 2B8
Target Species Mouse, Porcine
Conjugate PE-Cyanine5
Isotype IgG2b, kappa
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-140-90 Non-distribution product as customer accommodation.
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Invitrogen
15117181
50 μg
Each for $152.00
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50-140-91 Non-distribution product as customer accommodation.
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Invitrogen
15117182
100 μg
Each for $241.00
Add to cart
 
50-140-92 Non-distribution product as customer accommodation.
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Invitrogen
15117183
200 μg
Each for $358.00
Add to cart
 
Description

Description

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. Applications Reported: The 2B8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat.

00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. c-Kit, also known as CD117 and stem cell factor receptor, is a 145 kDa transmembrane tyrosine kinase encoded by the c-Kit proto-oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. c-Kit mutations correlate with tumor growth and progression in a variety of cancers including mast cell disease, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma, and lung cancer. Phosphorylation at tyrosine 703 of c-Kit allows binding of Grb2 and activation of the Ras-Raf-ERK1&2 signaling pathway.
Specifications

Specifications

CD117 (c-Kit)
Mouse, Porcine
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
2B8
PE-Cyanine5
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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