Invitrogen™ CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red

Rat Monoclonal Antibody

Supplier:  Invitrogen™ RM2817

Catalog No. 50-113-7581

Description

The M1/70.15 monoclonal antibody (mAb) reacts with the alpha-M subunit of Mac-1 (CD11b/CD18). For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm). Applications for the M1/70.15 mAb include immunoprecipitation, immunostaining for flow cytometry, IHC of acetone-fixed frozen sections, and blocking of CR3-mediated rosetting. Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Every lot is tested by flow cytometry using freshly harvested mouse blood.

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

Specifications

• Antigen: CD11b
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 4mg/mL BSA, sucrose and 0.1% sodium azide
• Gene Accession No.: P05555
• Gene Symbols: ITGAM
• Immunogen: C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry
• Clone: M1/70.15
• Conjugate: PE-Texas Red
• Gene: ITGAM
• Gene Alias: antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product
• Host Species: Rat
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 16409
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov