CD11b Mouse anti-Rat, FITC, Clone: WT.5, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 561684

Catalog No. BDB561684

Description

The WT.5 monoclonal antibody specifically binds to CD11b, the α subunit of Mac-1 found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD11b
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: Itgam; Integrin αM chain; Mac-1 α chain; Integrin beta 2 alpha subunit
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Rat
• Isotype: IgA κ

• Applications: Flow Cytometry
• Clone: WT.5
• Conjugate: FITC
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Rt neutrophils
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.