Invitrogen™ CD122 Monoclonal Antibody (5H4), PE, eBioscience™, Invitrogen™

Catalog No.	Quantity
12-122-182	100 μg
50-105-89	200 μg

Catalog No. 12-122-182 Supplier Invitrogen™ Supplier No. 12122182

Description

Rat Monoclonal Antibody

Description: The 5H4 antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain. CD122 is expressed by NK cells and some T cells in the periphery and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to CD122 is not inhibited by the 5H4 antibody. Applications Reported: The 5H4 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 5H4 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.

Specifications

• Antigen: CD122
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 5H4
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Il2rb
• Gene Accession No.: P16297
• Gene Alias: CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL15RB; IL15Rbeta; IL-15Rbeta; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; Il-2/15Rbeta; IL-2R subunit beta; IL2RB; IL-2RB; IL2RBC; Il-2Rbeta; interleukin 15 receptor, beta; interleukin 2 receptor subunit beta; interleukin 2 receptor, beta; interleukin 2 receptor, beta chain; Interleukin-15 receptor subunit beta; Interleukin-2 receptor subunit beta; p70; p70-75; p75
• Gene Symbols: Il2rb
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16185
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

For Research Use Only.