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CD127 Monoclonal Antibody (A7R34), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

$116.00

Specifications

Antigen CD127
Clone A7R34
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
46-127-180
Encompass_Preferred
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Invitrogen
46127180
25 μg
Each for $116.00
Add to Cart
 
46-127-182
Encompass_Preferred
View Documents
Invitrogen
46127182
100 μg
Each for $116.00
Edge
Add to Cart
 
Description

Description

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127. Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis. Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.
Specifications

Specifications

CD127
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide, pH 7.2
CD127, CD127 antigen, CDW127, IL 7 receptor, IL 7R a, IL 7R subunit alpha, IL 7R a, IL 7Ra, IL 7Ra, IL7 receptor, IL-7 receptor alpha chain, IL7 receptor subunit alpha, IL-7 receptor subunit alpha, IL7R, IL-7R, IL7R a, IL7R alpha, IL7R subunit alpha, IL-7R subunit alpha, IL7R a, IL7RA, IL-7RA, IL-7Ralpha, IL-7R-alpha, IL7Ra, ILRA, interleukin 7 receptor, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, Interleukin7 receptor subunit alpha, interleukin-7 receptor subunit alpha, MGC107557, sCD127, soluble IL 7 receptor, soluble IL 7R
Il7r
Primary
4° C, store in dark, DO NOT FREEZE!
Il7r
A7R34
Flow Cytometry
PerCP-eFluor 710
Rat
Mouse
P16872
16197
IgG2a κ
Affinity chromatography
Antibody
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