Invitrogen™ CD137 (4-1BB) Monoclonal Antibody (17B5), eFluor™ 450, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-9304	100 μg

Catalog No. 50-112-9304 Supplier Invitrogen™ Supplier No. 48137182

Description

Syrian Hamster Monoclonal Antibody

Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays. Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 17B5 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

Specifications

• Antigen: CD137 (4-1BB)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 17B5
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 450
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: TNFRSF9
• Gene Accession No.: P20334
• Gene Alias: 4 1 BB; 41BB; 4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; EGK_00183; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
• Gene Symbols: TNFRSF9
• Host Species: Syrian Hamster
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21942
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

For Research Use Only.