CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3867	Invitrogen 46590182	100 μg

Description

Description: The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction. Applications Reported: This TKS-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TKS-1 antibody has been tested by flow cytometric analysis of stimulated mouse spleen cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

CD137 Ligand (4-1BB Ligand) Monoclonal antibody specifically detects CD137 Ligand (4-1BB Ligand) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD137 Ligand (4-1BB Ligand)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P41274
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: TKS-1
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 21950
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.